Carter BloodCare has expertise in processing autologous and allogeneic HPCs, and bone marrow when a major or minor ABO incompatibility exists, to do CD34 selection, or other manipulations as requested.

The Cell Therapy Laboratory at Carter BloodCare will process autologous or allogeneic HPCs and bone marrow when a major or minor ABO incompatibility exists as necessary by manual, semi-automated or automated methods. They have the ability to process cord blood allografts supplied by NMDP public and private Cord Blood Banks.

The Cell Therapy Laboratory has the CliniMACs device for magnetic cell separation. Magnetic cell separation is used for CD34 selection of cellular therapy products, such as T-cell depletion.

The lab features board certified medical laboratory scientists who have received specialized training in HPC processing. Each HPC product is routinely evaluated for volume, cell viability, microbial contamination, TNC and TNC/kg, MNC%, total CD34 and CD34/kg, total CD3 and CD3/kg for allogeneic donors, and able to perform other evaluations upon request. Flow cytometry testing is done onsite for rapid turn-around time of results.

Cryopreservation

Cryopreservation is performed using a cryoprotectant solution containing 10% DMSO, an electrolyte solution (Plasma-Lyte A, pH 7.4), and a source of protein (25% human albumin). An equal amount of cryoprotectant and cells is added to each freezing bag. Bags are placed in freezing presses where they are monitored by a “ribbon probe” thermocouple. Bags are frozen using a controlled rate freezing system until the cells reach approximately -90°C.

Storage

Once cells are frozen they are removed from the freezing chamber, sealed in an overwrap bag, placed in an aluminum cassette, and stored in the vapor phase of liquid nitrogen at approximately -150°C or colder.

Storage agreements are for a period of two years. An annual storage fee is applied after the first year. If cells remain stored after the agreement expires the intended transplant recipient and the transplant physician are notified and must sign a Renewal Agreement for Continued Storage of HPCs.

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